Latest Microbial community profiling topics

Topic	Replies	Views	Activity
Absent tryptophan pathway in all metagenomic results HUMAnN	1	33	December 5, 2025
Humann=4.0.0a1 : MinPath12hmp.py:804: SyntaxWarning HUMAnN	1	51	December 5, 2025
Reverse forward reads as input in MetaPhlAn 4 MetaPhlAn	0	21	December 5, 2025
HUMAnN 3.0 stratified KOs return paradoxical interpretations HUMAnN	1	44	December 4, 2025
How to parallelize baqlava over many samples? BAQLaVa	2	30	December 4, 2025
Adding a --metaphlan-options and/or bowtie2db flag to baqlava BAQLaVa	2	35	December 4, 2025
MinPath error with baqlava 0.5 metaphlan 3.1.0 and humann 3.9 BAQLaVa	2	29	December 4, 2025
MetaPhlAn 4 + HUMAnN 4 compatibility HUMAnN HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…	10	924	November 30, 2025
Unable to merge virus profile.txt files into table MetaPhlAn	3	222	November 27, 2025
Euks in bt2 index but not in .fna? MetaPhlAn	0	19	November 24, 2025
Minimap2 Database Files for MetaPhlAn 4.2.2 MetaPhlAn	7	119	November 20, 2025
Humann biocontainer: Unable to write to the HUMAnN config file HUMAnN	3	772	November 14, 2025
RA fixing of vJun23_202307 DB MetaPhlAn	0	49	November 7, 2025
How should I compare HUMAnN 4 “Counts” from concatenated paired-end reads to known species read abundances? HUMAnN	3	89	November 6, 2025
Metaphlan Results Interpretation MetaPhlAn	2	61	November 5, 2025
🌓 PPANINI tool sunset notice: transitioning to MetaWIBELE PPANINI We are officially sunsetting PPANINI. PPANINI has been a valuable tool for protein family annotation and pan-genome analysis, but it has now been replaced by MetaWIBELE, a more comprehensive and actively maintained work…	1	18	November 4, 2025
Unable to download the databases in Humann4 HUMAnN	1	102	November 4, 2025
Optimal thread count for KneadData, Bowtie2, MetaPhlAn, and HUMAnN on multi-core systems HUMAnN	1	66	November 4, 2025
Very low mapping rate of Hummann v4alpha HUMAnN	1	65	November 4, 2025
Downloading humann from HPC cluster HUMAnN	1	48	November 4, 2025
Big differences in results depending on type of mapping used? HUMAnN	5	161	November 4, 2025
Humann3 does not recognize "relative abundance" in metaphlan profile file HUMAnN	8	1019	November 4, 2025
Discrepancies in prescreen.py script HUMAnN	1	18	November 4, 2025
Presence of fungi in ChocoPhlAn vJan25 MetaPhlAn	1	65	October 30, 2025
Metaphlan --install deletes minimap fasta MetaPhlAn	2	72	October 29, 2025
Allowing bowtie2 memory mapping in metaphlan MetaPhlAn	3	562	October 28, 2025
Connecting SGB ID with fasta of a MAG/genome? MetaPhlAn	0	46	October 28, 2025
Rarecurve on long read from Metaphlan 4.2.2 MetaPhlAn	3	63	October 27, 2025
Best practices for visualizing MetaPhlAn 4 MAG identifiers (SGB, FGB, etc.)? MetaPhlAn	1	46	October 27, 2025
MetaPhlan doesn't detect any microbial samples MetaPhlAn	2	47	October 27, 2025

Absent tryptophan pathway in all metagenomic results

HUMAnN

1

33

December 5, 2025

Humann=4.0.0a1 : MinPath12hmp.py:804: SyntaxWarning

HUMAnN

1

51

December 5, 2025

Reverse forward reads as input in MetaPhlAn 4

MetaPhlAn

0

21

December 5, 2025

HUMAnN 3.0 stratified KOs return paradoxical interpretations

HUMAnN

1

44

December 4, 2025

How to parallelize baqlava over many samples?

BAQLaVa

2

30

December 4, 2025

Adding a --metaphlan-options and/or bowtie2db flag to baqlava

BAQLaVa

2

35

December 4, 2025

MinPath error with baqlava 0.5 metaphlan 3.1.0 and humann 3.9

BAQLaVa

2

29

December 4, 2025

MetaPhlAn 4 + HUMAnN 4 compatibility

HUMAnN

HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…

10

924

November 30, 2025

Unable to merge virus profile.txt files into table

MetaPhlAn

3

222

November 27, 2025

Euks in bt2 index but not in .fna?

MetaPhlAn

0

19

November 24, 2025

Minimap2 Database Files for MetaPhlAn 4.2.2

MetaPhlAn

7

119

November 20, 2025

Humann biocontainer: Unable to write to the HUMAnN config file

HUMAnN

3

772

November 14, 2025

RA fixing of vJun23_202307 DB

MetaPhlAn

0

49

November 7, 2025

How should I compare HUMAnN 4 “Counts” from concatenated paired-end reads to known species read abundances?

HUMAnN

3

89

November 6, 2025

Metaphlan Results Interpretation

MetaPhlAn

2

61

November 5, 2025

🌓 PPANINI tool sunset notice: transitioning to MetaWIBELE

PPANINI

We are officially sunsetting PPANINI. PPANINI has been a valuable tool for protein family annotation and pan-genome analysis, but it has now been replaced by MetaWIBELE, a more comprehensive and actively maintained work…