Trouble running HUMAnN with updated metaphlan database

Microbial community profiling HUMAnN
1

Hi all,

I’m trying to run HUMAnN 3.8 with the latest MetaPhlAn v4 database, but I keep running into an error during the taxonomic profiling step. It says the MetaPhlAn version is incompatible, even though I’ve updated both tools via conda and confirmed the database is downloaded.

Has anyone successfully configured HUMAnN to work with MetaPhlAn 4.x? Do I need to stick with MetaPhlAn 3 for now, or is there a way to bypass the compatibility check?

Any help or guidance would be greatly appreciated. Let me know if I should share logs or version details.

Thanks in advance!
Jhonn Mick

2

The different HUMAnN 3.x releases are each compatible with a separate MetaPhlAn 4 database (please see their release notes for details). However, we have not released a HUMAnN 3.x yet for the latest (Jan25) MetaPhlAn 4 database, and I’m not sure if we will given that we are shifting focus to HUMAnN 4 at this point.

You and others here are welcome to reply and let us know if maintaining backward compatibility is important for your workflows. If there’s a lot of interest that would provide more motivation for another HUMAnN 3.x release.