HUMAnN

Topic	Replies	Views	Activity
MetaPhlAn 4 + HUMAnN 4 compatibility HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…	23	1719	March 27, 2026
Announcing HUMAnN 4.0 (alpha) Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…	4	3149	February 2, 2026
Custom MetaPhlAn subspecies taxonomy + corresponding HUMAnN ChocoPhlAn pangenome design	1	2	April 29, 2026
What’s is needed to build a custom HUMANN database?	7	1049	April 29, 2026
Everything is unclassified	0	2	April 28, 2026
Unable to download Databases	10	28	April 23, 2026
Metaphlan 4 using with Humann3.9	5	429	April 21, 2026
Can't download Uniref database	0	7	April 20, 2026
Humann_unpack_pathways command	5	236	April 15, 2026
Matching tools and database versions	8	152	April 9, 2026
Tutorial not up to date and unable to run successfully	0	26	April 5, 2026
No full uniref90 db in humann4	2	91	March 30, 2026
Humann 3.9 conda install will not work. Please update your version of MetaPhlAn?	6	314	March 9, 2026
Humann3 hanging during run, no error messages but no changes to output, log or temp files	21	1055	March 9, 2026
Very low mapping rate of Hummann v4alpha	3	131	March 6, 2026
Latest Stable version of HUMANn and MetaPhlAN for maximum downstream compatibility	0	131	March 3, 2026
Unable to download ChocoPhlAn and UniRef databases (connection error)	2	48	March 2, 2026
Best publication to referenec when citing humann4	0	23	February 26, 2026
Humann3 --metaphlan-options error	0	19	February 18, 2026
READS_UNMAPPED Is Not Presented in CPM Format	2	128	February 18, 2026
Dereplicating and normalizing reads for humann and metaphlan	6	97	January 30, 2026
Failure Downloading HUMAnN UniRef90 DIAMOND Database via WSL	0	11	January 28, 2026
Glitch in humann4 genefamilies file creation	0	26	January 15, 2026
No SGBs in humann3.9 genefamily.tsv	2	34	January 12, 2026
Pathway abundance stratification	0	35	January 9, 2026
How to handle transferred and split EC numbers in humann outputs	1	19	January 9, 2026
HUMAnN targeted functional analysis?	2	98	January 9, 2026
Humann (v3.9) returning 100% unaligned reads on demo data	1	42	January 9, 2026
HUMAnN4 Support for MetaPhlAn vJan25 Database	1	127	January 9, 2026
HUMAnN 4 database downloads inaccessible due to Globus (data.globus.org) domain blocking	2	66	December 9, 2025

MetaPhlAn 4 + HUMAnN 4 compatibility

HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…

23

1719

March 27, 2026

Announcing HUMAnN 4.0 (alpha)

Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…