HUMAnN

Topic	Replies	Views	Activity
MetaPhlAn 4 + HUMAnN 4 compatibility HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…	23	1577	March 27, 2026
Announcing HUMAnN 4.0 (alpha) Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…	4	3026	February 2, 2026
Matching tools and database versions	8	116	April 9, 2026
Metaphlan 4 using with Humann3.9	2	352	April 9, 2026
Tutorial not up to date and unable to run successfully	0	12	April 5, 2026
No full uniref90 db in humann4	2	74	March 30, 2026
Humann 3.9 conda install will not work. Please update your version of MetaPhlAn?	6	284	March 9, 2026
Humann3 hanging during run, no error messages but no changes to output, log or temp files	21	1053	March 9, 2026
Very low mapping rate of Hummann v4alpha	3	125	March 6, 2026
Latest Stable version of HUMANn and MetaPhlAN for maximum downstream compatibility	0	102	March 3, 2026
Unable to download ChocoPhlAn and UniRef databases (connection error)	2	43	March 2, 2026
Best publication to referenec when citing humann4	0	15	February 26, 2026
Humann3 --metaphlan-options error	0	13	February 18, 2026
READS_UNMAPPED Is Not Presented in CPM Format	2	120	February 18, 2026
Dereplicating and normalizing reads for humann and metaphlan	6	91	January 30, 2026
Failure Downloading HUMAnN UniRef90 DIAMOND Database via WSL	0	10	January 28, 2026
Glitch in humann4 genefamilies file creation	0	26	January 15, 2026
No SGBs in humann3.9 genefamily.tsv	2	34	January 12, 2026
Pathway abundance stratification	0	33	January 9, 2026
How to handle transferred and split EC numbers in humann outputs	1	18	January 9, 2026
HUMAnN targeted functional analysis?	2	94	January 9, 2026
Humann (v3.9) returning 100% unaligned reads on demo data	1	42	January 9, 2026
HUMAnN4 Support for MetaPhlAn vJan25 Database	1	125	January 9, 2026
HUMAnN 4 database downloads inaccessible due to Globus (data.globus.org) domain blocking	2	62	December 9, 2025
Humann3 Database errors	7	178	December 8, 2025
HUMAnN (v3.6) a new db update issue - CHOCOPhlAnSGB_202103	6	166	December 7, 2025
How to silence database download message from Metaphlan 4.0.6 within Humann 3.8	4	165	December 7, 2025
HUMAnN 4 prescreen issue and input file format clarification	2	70	December 7, 2025
Combine Output from Separate Runs of Single Sample	3	74	December 5, 2025
Trouble running HUMAnN with updated metaphlan database	3	260	December 5, 2025

MetaPhlAn 4 + HUMAnN 4 compatibility

HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…

23

1577

March 27, 2026

Announcing HUMAnN 4.0 (alpha)

Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…