HUMAnN

Topic	Replies	Views	Activity
MetaPhlAn 4 + HUMAnN 4 compatibility HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…	23	1846	March 27, 2026
Announcing HUMAnN 4.0 (alpha) Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…	4	3274	February 2, 2026
HUMAnN v4 alpha: clarification on 0.5x coverage threshold and low-abundance taxa filtering	1	5	May 22, 2026
HUMAnN v4.0.0.alpha.1 Raw Counts	1	20	May 20, 2026
Humann4 conda install from release note	1	35	May 14, 2026
Everything is unclassified	3	25	May 1, 2026
Custom MetaPhlAn subspecies taxonomy + corresponding HUMAnN ChocoPhlAn pangenome design	1	18	April 29, 2026
What’s is needed to build a custom HUMANN database?	7	1067	April 29, 2026
Unable to download Databases	10	55	April 23, 2026
Metaphlan 4 using with Humann3.9	5	488	April 21, 2026
Can't download Uniref database	0	12	April 20, 2026
Humann_unpack_pathways command	5	247	April 15, 2026
Matching tools and database versions	8	164	April 9, 2026
Tutorial not up to date and unable to run successfully	0	37	April 5, 2026
No full uniref90 db in humann4	2	119	March 30, 2026
Humann 3.9 conda install will not work. Please update your version of MetaPhlAn?	6	339	March 9, 2026
Humann3 hanging during run, no error messages but no changes to output, log or temp files	21	1057	March 9, 2026
Very low mapping rate of Hummann v4alpha	3	138	March 6, 2026
Latest Stable version of HUMANn and MetaPhlAN for maximum downstream compatibility	0	163	March 3, 2026
Unable to download ChocoPhlAn and UniRef databases (connection error)	2	55	March 2, 2026
Best publication to referenec when citing humann4	0	31	February 26, 2026
Analyzing single end and paired end reads together?	0	12	February 20, 2026
Humann3 --metaphlan-options error	0	23	February 18, 2026
READS_UNMAPPED Is Not Presented in CPM Format	2	138	February 18, 2026
Dereplicating and normalizing reads for humann and metaphlan	6	104	January 30, 2026
Failure Downloading HUMAnN UniRef90 DIAMOND Database via WSL	0	13	January 28, 2026
Glitch in humann4 genefamilies file creation	0	27	January 15, 2026
No SGBs in humann3.9 genefamily.tsv	2	35	January 12, 2026
Pathway abundance stratification	0	37	January 9, 2026
How to handle transferred and split EC numbers in humann outputs	1	20	January 9, 2026

MetaPhlAn 4 + HUMAnN 4 compatibility

HUMAnN users - I’m making a quick pinned post about MetaPhlAn 4 + HUMAnN 4 compatibility since we’re seeing a lot of posts raising similar issues. We will follow up with more detailed information in the short term, and l…

23

1846

March 27, 2026

Announcing HUMAnN 4.0 (alpha)

Dear HUMAnN users: HUMAnN 4.0 (alpha) has arrived! Many thanks for your patience—we know there have been a lot of users looking forward to this release for a long time. Since a lot is changing in this version, we’re tryi…