Hi,
As the title says, I tried running humann3 on the demo data but found 100% unaligned reads. I’m guessing that this shouldn’t be the case?
My command is:
humann --input demo.fastq.gz --output demo_fastq
The output is:
Running bowtie2 …
Total bugs from nucleotide alignment: 0
Total gene families from nucleotide alignment: 0
Unaligned reads after nucleotide alignment: 100.0000000000 %
Running diamond …
Aligning to reference database: uniref90_201901b_full.dmnd
CRITICAL ERROR: Error executing: /home/user/miniconda3/envs/humann3/bin/diamond blastx --query /home/user/metagenomics/results/humann_demo/demo_humann_temp/demo_bowtie2_unaligned.fa --evalue 1.0 --threads 1 --top 1 --outfmt 6 --db /home/user/metagenomics/databases/humann/uniref/uniref90_201901b_full --out /home/user/metagenomics/results/humann_demo/demo_humann_temp/tmpja7rf3jq/diamond_m8_rm_2z37c --tmpdir /home/user/metagenomics/results/humann_demo/demo_humann_temp/tmpja7rf3jq
Would greatly appreciate any help or advice. Thanks!