Hi @fbeghini, @franzosa !!!
I am getting 67.78% unaligned reads with HUMAnN 3.0 after nucleotide search tier. After the translated search (against uniref90_ec_filtered database) it reduced to 63.05%.
Is it very high amount and unlikely? If yes, what should I do?
What is a normal range of unaligned reads for this step?