Inquiry regarding the compatible ChocoPhlAn database version for MetaPhlAn 4.2.4 and StrainPhlAn 4.1

Microbial community profiling StrainPhlAn
1

Hi,

I am a graduate student researching the human microbiome. I am currently using MetaPhlAn 4.2.4 and StrainPhlAn 4.1 on a high-performance computing cluster without direct internet connection.

I would like to clarify which ChocoPhlAn database version is most recommended for these specific tool versions. Currently, I have mpa_vOct22_CHOCOPhlAnSGB_202212 installed and it seems to be working. However, I noticed that the latest version available on the server is mpa_vJan25_CHOCOPhlAnSGB_202503.

My questions are:

  1. Is MetaPhlAn 4.2.4 fully compatible with the vJan25 database?

  2. Does StrainPhlAn 4.1 require a specific database version to ensure the best results for strain-level analysis?

  3. Should I stick with the vOct22 version, or is it highly recommended to manually update to vJan25?

Thank you for your help!

Best regards, [Your Name]