Greetings!
I tried to construct a tree of life using the second example of Phylophlan (I use 15 genomes for a trial). And the output_tol folder only contains a temp fold, which was further divided by two folders: “clean_dna” and “map_dna”. The documents in the “map_dna” is all blank, and the code is still running without stopping. Please help me solve this problem.
base) winger@880729:~/examples/02_tol$ phylophlan
-i input_genomes
-d phylophlan
-f 02_tol.cfg
–diversity low
–accurate
-o output_tol
–nproc 4
–verbose 2>&1 | tee logs/phylophlan.log
tee: logs/phylophlan.log: 没有那个文件或目录
PhyloPhlAn version 3.0.60 (27 November 2020)
Command line: /home/winger/miniconda3/bin/phylophlan -i input_genomes -d phylophlan -f 02_tol.cfg --diversity low --accurate -o output_tol --nproc 4 --verbose
Automatically setting “input=input_genomes” and “input_folder=/home/winger/examples/02_tol”
[e] “/home/winger/miniconda3/lib/python3.7/site-packages/phylophlan/phylophlan_configs/” folder does not exists
Creating folder “output_tol”
Creating folder “output_tol/tmp”
“low-accurate” preset
Setting “sort=True” because “database=phylophlan”
Setting “min_num_markers=100” since no value has been specified and the “database=phylophlan”
Arguments: {‘input’: ‘input_genomes’, ‘clean’: None, ‘output’: ‘output_tol’, ‘database’: ‘phylophlan’, ‘db_type’: None, ‘config_file’: ‘02_tol.cfg’, ‘diversity’: ‘low’, ‘accurate’: True, ‘fast’: False, ‘clean_all’: False, ‘database_list’: False, ‘submat’: ‘pfasum60’, ‘submat_list’: False, ‘submod_list’: False, ‘nproc’: 4, ‘min_num_proteins’: 1, ‘min_len_protein’: 50, ‘min_num_markers’: 100, ‘trim’: ‘not_variant’, ‘gap_perc_threshold’: 0.67, ‘not_variant_threshold’: 0.99, ‘subsample’: None, ‘unknown_fraction’: 0.3, ‘scoring_function’: None, ‘sort’: True, ‘remove_fragmentary_entries’: False, ‘fragmentary_threshold’: 0.85, ‘min_num_entries’: 4, ‘maas’: None, ‘remove_only_gaps_entries’: False, ‘mutation_rates’: False, ‘force_nucleotides’: False, ‘input_folder’: ‘/home/winger/examples/02_tol/input_genomes’, ‘data_folder’: ‘output_tol/tmp’, ‘databases_folder’: ‘phylophlan_databases/’, ‘submat_folder’: ‘/home/winger/miniconda3/lib/python3.7/site-packages/phylophlan/phylophlan_substitution_matrices/’, ‘submod_folder’: ‘/home/winger/miniconda3/lib/python3.7/site-packages/phylophlan/phylophlan_substitution_models/’, ‘configs_folder’: None, ‘output_folder’: ‘’, ‘genome_extension’: ‘.fna’, ‘proteome_extension’: ‘.faa’, ‘update’: False, ‘verbose’: True}
Loading configuration file “02_tol.cfg”
Checking configuration file
Checking “/home/winger/miniconda3/bin/diamond”
Checking “/home/winger/miniconda3/bin/mafft”
Checking “/home/winger/miniconda3/bin/trimal”
Checking “/home/winger/miniconda3/bin/iqtree”
“db_aa” database “phylophlan_databases/phylophlan/phylophlan.dmnd” present
Loading files from “/home/winger/examples/02_tol/input_genomes”
Creating folder “output_tol/tmp/clean_dna”
Cleaning 15 inputs
Cleaning “/home/winger/examples/02_tol/input_genomes/Acetatifactor_muris.fna”
Cleaning “/home/winger/examples/02_tol/input_genomes/Acutalibacter_muris.fna”
Cleaning “/home/winger/examples/02_tol/input_genomes/Adlercreutzia_equolifaciens.fna”
Cleaning “/home/winger/examples/02_tol/input_genomes/Akkermansia_muciniphila.fna”
“output_tol/tmp/clean_dna/Akkermansia_muciniphila.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Akkermansia_muciniphila_CAG_154.fna”
“output_tol/tmp/clean_dna/Adlercreutzia_equolifaciens.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_communis.fna”
“output_tol/tmp/clean_dna/Acutalibacter_muris.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_putredinis.fna”
“output_tol/tmp/clean_dna/Akkermansia_muciniphila_CAG_154.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_sp_An31A.fna”
“output_tol/tmp/clean_dna/Acetatifactor_muris.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_sp_CAG_268.fna”
“output_tol/tmp/clean_dna/Alistipes_putredinis.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_sp_CHKCI003.fna”
“output_tol/tmp/clean_dna/Alistipes_communis.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alistipes_timonensis_JC136.fna”
“output_tol/tmp/clean_dna/Alistipes_sp_An31A.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Alloprevotella_rava.fna”
“output_tol/tmp/clean_dna/Alistipes_sp_CHKCI003.fna” generated in 0s
“output_tol/tmp/clean_dna/Alistipes_sp_CAG_268.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Anaerotruncus_sp_G3_2012.fna”
Cleaning “/home/winger/examples/02_tol/input_genomes/Atopobium_minutum.fna”
“output_tol/tmp/clean_dna/Alistipes_timonensis_JC136.fna” generated in 0s
Cleaning “/home/winger/examples/02_tol/input_genomes/Bacteroides_caccae.fna”
“output_tol/tmp/clean_dna/Atopobium_minutum.fna” generated in 0s
“output_tol/tmp/clean_dna/Alloprevotella_rava.fna” generated in 0s
“output_tol/tmp/clean_dna/Anaerotruncus_sp_G3_2012.fna” generated in 0s
“output_tol/tmp/clean_dna/Bacteroides_caccae.fna” generated in 0s
Loading files from “output_tol/tmp/clean_dna”
Creating folder “output_tol/tmp/map_dna”
Mapping “phylophlan” on 15 inputs (key: “map_dna”)
Mapping “output_tol/tmp/clean_dna/Acetatifactor_muris.fna”
Mapping “output_tol/tmp/clean_dna/Acutalibacter_muris.fna”
Mapping “output_tol/tmp/clean_dna/Adlercreutzia_equolifaciens.fna”
Mapping “output_tol/tmp/clean_dna/Akkermansia_muciniphila.fna”
Thank you so much.