The bioBakery help forum

LEfSe input won't let me choose class row


The LEfSe tool doesn’t recognise my rows, it seems. The input file is a tabs separated txt file. As a result, I can’t choose a class row, making it impossible to use the LEfSe tool.
With the example file it works fine! What is wrong with my file?

With kind regards,


I’m not sure what’s causing the error. FWIW I created a toy file mimicking ASV_lefse_asv_oneclass.txt in your screenshot, and it works okay. Could you share the input through email ( Feel free to mask sensitive data.