I’m trying to run the first step of LEfSe microbiome analysis from Huttenhower lab on the left menu, running:
LEfSe: A) Format data for LEfSe
I’m getting the following error for the last two days:
“Tool request failed
Uncaught exception in the exposed API method:”
It worked just fine last week. Can you please let me know how to correct this issue?
Many thanks in advance
Hi Nevenka !
Can you try to run the sample lefse file that we provide and let me know if it works ?
I am posting the file gere, just in case …
Also, how big is the file that you are trying to process?
And: Can you compare the internal structure of your file to the sample file we provide ?
Let me know,
George Weingart PhD
73.txt (82.9 KB)
I saw two problems:
- The third row does not have all the columns
- The row names have some problem - for example - row 61 has double quotes.
I replaced the rownames with generic a1…a101 and ran it successfully.
Hope this helps.
Enclosed a text file that works as well as screen print of a successful run.
test2.txt (146.2 KB)
Thank you!! I will check all the names again, and fix it they appeared fine in Excel.