Humann 3.8 update caused metaphlan error

Microbial community profiling HUMAnN
1

Hello,

I updated my version to your new Humann 3.8 release and now I am met with this error:

ERROR: The MetaPhlAn taxonomic profile provided was not generated with the database version v3 or vOct22 . Please update your version of MetaPhlAn to at least v3.0 or if you are using MetaPhlAn v4 please use the database vOct22. site:forum.biobakery.org

I see that this has been a very common error in the past, but it had been resolved for me when I was previously using Humann3.7

These are my current versions:

humann --version
humann v3.8

metaphlan --version
MetaPhlAn version 4.0.6 (1 Mar 2023)

2

Thank you for the detailed post. Can you also post which version of the MetaPhlAn v4 database you are using?

Thanks!
Lauren

3

Hi Lauren,

Thanks for your response!

I fixed the issue by downloading the metaphlan database separately using metaphlan --install, and then I specified the location of the metaphlan database while running Humann3 by using --metaphlann-options “–bowtie2db /path/to/db”

Not sure if this was just an issue for me based on my installation, but hopefully this helps other people in case they run into the same problem!.

Thanks,
J

1 Like
4

Hi @jkcopes , Thanks for the follow up! Glad to hear you are set.

Thanks!
Lauren