Humann 3.8 update caused metaphlan error


I updated my version to your new Humann 3.8 release and now I am met with this error:

ERROR: The MetaPhlAn taxonomic profile provided was not generated with the database version v3 or vOct22 . Please update your version of MetaPhlAn to at least v3.0 or if you are using MetaPhlAn v4 please use the database vOct22.

I see that this has been a very common error in the past, but it had been resolved for me when I was previously using Humann3.7

These are my current versions:

humann --version
humann v3.8

metaphlan --version
MetaPhlAn version 4.0.6 (1 Mar 2023)

Thank you for the detailed post. Can you also post which version of the MetaPhlAn v4 database you are using?


Hi Lauren,

Thanks for your response!

I fixed the issue by downloading the metaphlan database separately using metaphlan --install, and then I specified the location of the metaphlan database while running Humann3 by using --metaphlann-options “–bowtie2db /path/to/db”

Not sure if this was just an issue for me based on my installation, but hopefully this helps other people in case they run into the same problem!.


Hi @jkcopes , Thanks for the follow up! Glad to hear you are set.