I run HUMAnN3.5 with MetaPhlAn4 in linux but get a error, here is error message in screen:
ERROR: The MetaPhlAn taxonomic profile provided was not generated with the expected database version. Please update your version of MetaPhlAn to at least v3.0.
These two software were installed by conda, an HUMAnN version is v3.5, MetaPhlAn version is 4.0.1 (24 Aug 2022), the database were download and config using humann_database and metaphlan --install. my command line was pasted below:
humann -i merge.fq -o sample --threads 30 --search-mode uniref90 --memory-use maximum --metaphlan-options “-t rel_ab_w_read_stats” --pathways unipathway
please tell me what’s wrong, I can’t fix it by myself.
PS. I have run humann_test and all thing is ok. I also have run MetaPhlAn4 separately, and it correctly worked, and same data was successfully tested using HUMAnN3.1 with MetaPhlAn3