CRITICAL ERROR: The metaphlan executable can not be found. Please check the install

Microbial community profiling HUMAnN
1

Dear all,

I’m trying to properly install humann3 using Conda environment. I’ve followed these instructions: humann3 – The Huttenhower Lab

But when I want to run the HUMAnN demo:

humann -i demo.fastq.gz -o reference/sample_results/

It doesn’t work and a error message appears:

Output files will be written to: ~/reference/sample_results
Decompressing gzipped file ...

CRITICAL ERROR: The metaphlan executable can not be found. Please check the install.

I didn’t find any suggestion what to do. The test was ok.

Thank you!

2

Are you sure you have MetaPhlAn installed correctly as well? Are you able to call / run MetaPhlAn outside of HUMAnN?