Hello BioBakery Forum,
I was under the impression that shotgun metatranscriptome needs to couple with shotgun metagenome to complete the analysis Best practices for analysing microbiomes | Nature Reviews Microbiology. However, the Humann pipeline generated a MetaPhlAn table from the metatranscriptome run together with the pathway data.
I am just wondering can we use the metaphlan table generated in Humann3 metatranscriptome run as the community taxonomy reference? We have sequenced another set of metagenome data that generated the same files through Humann3 pipeline.
Thank you!!
Best,
Yike