When using MetaPhlAn 4.2.2 for species annotation under a WSL environment (with Ubuntu, a Linux system, set up on Windows via Miniconda), the process generates a 0KB compressed file named B1_bowtie2.BZ2. After running for a period of time, Ubuntu automatically crashes. The database has already been downloaded, and the --tmp_dir parameter was explicitly set to a non-C drive to ensure sufficient memory. Could anyone help analyze the possible causes of this issue?
Hi @Wen1
How much RAM did you provide to your environment? Depending on the database version you are using you may need large amounts of RAM to allow to load in memory the bowtie2 indexes (latest database vJan25 can be up to 30GB), so if not enough memory is provided the mapping may crash.