Running a single FASTQ sample has an empty output file

[Heba_Allah_Mohamed_H]

I installed metaPhlan in new environment using conda

then downloaded the tutrials files

I tried to run the command metaphlan SRS014476-Supragingival_plaque.fasta.gz --input_type fasta > SRS014476-Supragingival_plaque_profile.txt

but it gives me one empty file

I found i didn’t download the database so i used thid command to download it

$ metaphlan --install --bowtie2db dbFolder SRS014476-Supragingival_plaque.fasta.gz --input_type fasta > SRS014476-Supragingival_plaque_profile.txt

and this folder was created with total 26.9 GB

but still the output is the previous empty folder, Anybody can help me plz?

[aitor.blancomiguez]

Hi @Heba_Allah_Mohamed_H
It seems your machine does not have enough RAM memory to build the indexes, you can find here the already prebuilt ones: Index of /biobakery4/metaphlan_databases/bowtie2_indexes