Hello everyone,
I am interested in running Metaphlan4 and have been following the tutorial from Github. After having some issues installing the databases and bowtie2 indexes, I was able to run it. However, I noted that the results in my profile file differed in bacteria composition from the ones in the tutorial (please see attached file). The bowtieout file was also different (see attached). I was using this file SRS014476-Supragingival_plaque.fasta.gz and this is the command I used : $metaphlan SRS014476-Supragingival_plaque.fasta.gz --input_type fasta > SRS014476-Supragingival_plaque_profile.txt.
Could this result be a product of a problem with the database?
Could it be that the version I am using (4.06) is not compatible with the database (mpa_vJun23_CHOCOPhlAnSGB_202403)?
Thank you in advance for your attention.
SRS014476-Supragingival_plaque.fasta.gz.bowtie2out_test1.txt (53.5 KB)