Unable to find the mpa_pkl

[SofiaMurga]

Hi, I’m trying to run humann2 locally (macOS), I understand that proper dependencies should be installed using ‘conda install -c biobakery humann2’. In my case I got humann2 v2.8.1, MetaPhlAn version 2.6.0, diamond version 0.8.36, and Bowtie 2 version 2.3.5.1
I am trying to run the demo but I’m gettin the next error:
Error message returned from metaphlan2.py :
Error: Unable to find the mpa_pkl file at: //anaconda3/envs/py27/bin/db_v20/mpa_v20_m200.pkl
Expecting location ${mpa_dir}/db_v20/map_v20_m200.pkl
Select the file location with the option --mpa_pkl.

If I am understanding this correctly, I’m missing a database that should’ve been download with the Metaphlan package?
I thank you in advance!

[lauren.j.mciver]

Hello, Yes you are right. It looks like you do not have the MetaPhlAn database installed. Try removing all of the files in the expected database folder (on your system this looks to be //anaconda3/envs/py27/bin/db_v20/*) and then rerun the demo. MetaPhlAn should automatically install the database.

Thank you,
Lauren