Here’s the revised version with improved grammar and clarity:
Hi, I haven’t used Galaxy LEfSe analysis for a while, and I recently tried to use it again. However, I’m unable to proceed beyond the LDA Effect Size step. I even tested an old text file that worked perfectly a couple of years ago, but now, the same file is not working, and I keep encountering an error message. I’m not sure what I might be missing.
I’ve included a link to my text file, and I would greatly appreciate it if anyone could help me resolve this issue. Thank you!