It is my first time using LEfSe.
When I use the LEfSe tool online via http://galaxy.biobakery.org/root?tool_id=LEfSe_for I get the following error "Error: Cannot count lines in dataset format: lefse_internal_for, database:?
ERROR:galaxy.datatypes.data:Unable to count lines in file/export/galaxy-central/database/files/016/dataset_16921.
This error occurs even when I use the example data from galaxy-upgrade/galaxy_lefse/hmp_aerobiosis_small.txt at main · biobakery/galaxy-upgrade · GitHub as well as when I use my own data
output.txt (744.2 KB)
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I uploaded the data via A) Format Data for LEfSe (Galaxy Version 1.0) → Upload → Choose local files → hmp_aerobiosis_small.txt we do not specify the type parameter or the Genome parameter. Should we? Then we press the “Start” button and then the “Select”
Then I got the error I explained.
What is the problem? How can I fix it?
Thanks:)