The bioBakery help forum

16S raw data adaptors / primers

I’m wondering if the 16S raw datafiles have had adaptors and primers trimmed?

Hi Kristin,

Apologies for the late reply. I ran the FASTQC for one of the 16S raw data files and it seems that the adaptors and the primers are being trimmed. However, since it is RNA-Seq data, therefore there may be some transcripts that are so abundant that they register as overrepresented sequence.