Super basic query about taxa file for Maaslin3

Downstream analysis and statistics MaAsLin
1

Hi. I have species level shotgun data. I am conducting the following basic transformations.

- Converting count to relative abundance

- Dropping species with <10% prevalence in my sample

Are these the right steps to do before I setup my data? Is there any other important step that I am missing?

Thanks.

Sanyog

2

Hi Sanyog,

Both MaAsSLin 2 and MaAsLin 3 will automatically convert your counts to relative abundances, and our advice in MaAsLin 3 is to not drop the low prevalence species (though in MaAsLin 2 dropping those below 10% prevalence was the default and implemented automatically).

Will