🧬 Join us in Boston for the 2026 bioBakery Workshop
(Cambridge, MA · July 20–24) —
Learn more & apply
🔬 We’re also delighted to welcome you to the
8th Annual HCMPH Symposium
(May 18, 2026) —
Translating the Microbiome: Turning Discovery into Implementation
View symposium details
Hi @wfgui - we do not recommend any particular threshold but we observed that higher RTSI scores corresponded to better prediction accuracy in our dataset (Supplementary Figure 7 in the original manuscript). So please choose an arbitrary but not-unreasonable threshold as you feel appropriate for your dataset.