The bioBakery help forum

How to interpret RTSI value

Hi all,

What’s the cutoff of RTSI when using MelonnPan? In other words, what RTSI value means the sample is well represented by the training model?

Hi @nightkid - we do not have a recommended cutoff for the RTSI. As described in our manuscript, RTSI scores are computed by sequentially seeking the highest correlation coefficient with the top principal components (PCs) explaining a majority of the variation in the training microbiomes, which means it can be interpreted similarly to a correlation coefficient.