Sample2markers.py faced Error

Microbial community profiling StrainPhlAn
1

Hi, While I was using the Metaphlan4.1.1 sample2markers.py to extract each sample’s marker to the consensus marker directory. The problem occurred. Could you please help me solve that. The database is the latest version.

sample2markers.py -i *.sam.bz2 -o consensus_markers -n 10
Tue Jun 11 18:48:21 2024: Start samples to markers execution
Tue Jun 11 18:48:21 2024: Using samtools version 1.13
Tue Jun 11 18:48:21 2024: Creating temporary directory…
Tue Jun 11 18:48:21 2024: Done.
Tue Jun 11 18:48:21 2024: Filtering SAM files…
Tue Jun 11 18:48:21 2024: Loading MetaPhlAn mpa_vOct22_CHOCOPhlAnSGB_202403 database…
Tue Jun 11 18:48:56 2024: Done.
Tue Jun 11 18:49:34 2024: [Error] Marker UniClust90_MANJGFMI01329|1__8|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:34 2024: Stop execution.
Tue Jun 11 18:49:39 2024: [Error] Marker UniClust90_MANJGFMI01329|1__8|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:39 2024: Stop execution.
Tue Jun 11 18:49:43 2024: [Error] Marker UniRef90_A5YTT9|1__4|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:43 2024: Stop execution.
Tue Jun 11 18:49:48 2024: [Error] Marker UniRef90_D4FSC7|1__14|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:48 2024: Stop execution.
Tue Jun 11 18:49:52 2024: [Error] Marker UniRef90_I0Q4X7|1__15|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:52 2024: Stop execution.
Tue Jun 11 18:49:56 2024: [Error] Marker UniRef90_A5YTT9|1__4|SGB8130 not in the metaphlan database
Tue Jun 11 18:49:56 2024: Stop execution.

2

I have met the same problem, and my steps are same as the tutorial in git hub. My sam files look like

> FP180000019BRL1C015R02000021589/1__1.92 16 UniRef90_A0A2W5IBW0|1__6|SGB3663 280 42 150M * 0 0 CCCGAGTGGATACAACCTGACGCCGCAGACAATATATACGCATTTGCACAGGGGCCATCTGGCGCTTTCGCAGGTTTTGCACATTGGCATCACGGTAAATTAGAACGTGCTTTCACCGCGACAACCTGCGAAATTCTGGAGGATAAAGGG FFFFFFFF888F88,FF8F,8F88F888FF8F888FFF8FFFFFF88F8FFFFFF8FF8F8F8FFFFF8F8FFFFFFFFFFFFF8FFFFF8F8FFF8F8F888FF8FFFFFFF8FF8FFFF8FFFFFFF8F8FFFFFFF8FFF8888FFF AS:i:0 XN:i:0 XM:i:0 XO:i:0 XG:i:0 NM:i:0 MD:Z:150 YT:Z:UU

they have the same format with the id in “mpa_vOct22_CHOCOPhlAnSGB_202403_marker_info.txt” file ,and I’m sure use mpa_vOct22_CHOCOPhlAnSGB_202403 version of database for both metaphlan and samples2markers.py. How can I fix it?
can anyone help me ?