Missing Legend in Lefse Cladogram Plot Generated via Conda Environment

Hello.
I am currently using lefse_plot_cladogram.py within a conda environment to generate cladogram plots from LEfSe output files. While the cladogram images are generated successfully, the group/class legends do not appear on the output plots.

Could you kindly advise on the following points?

  • Are there specific options or settings to enable or control legend display in lefse_plot_cladogram.py?
  • Is this a known issue or are there any recommended workarounds?
  • For reference, the command I used is as follows:

lefse_plot_cladogram.py input.res output.png --format png --dpi 300

I am running LEfSe installed via conda with Python 3.12.

Thank you very much for your assistance. I look forward to your reply.

Best regards,
Takuto Kurakawa