Metaphlan4 BrokenPipeError

dragon@ser7:~/data/myfold$ metaphlan --bowtie2db /home/dragon/data/ sample.fasta --bowtie2out 4_bowtie2.bz2 --nproc 5 --input_type fasta --unclassified_estimation -o 4.txt
Killed
(ERR): bowtie2-align exited with value 137
Traceback (most recent call last):
File “/home/dragon/soft/anaconda3/envs/metaphlan/bin/read_fastx.py”, line 10, in
sys.exit(main())
File “/home/dragon/soft/anaconda3/envs/metaphlan/lib/python3.7/site-packages/metaphlan/utils/read_fastx.py”, line 168, in main
f_nreads, f_avg_read_length = read_and_write_raw(f, opened=False, min_len=min_len, prefix_id=prefix_id)
File “/home/dragon/soft/anaconda3/envs/metaphlan/lib/python3.7/site-packages/metaphlan/utils/read_fastx.py”, line 130, in read_and_write_raw
nreads, avg_read_length = read_and_write_raw_int(inf, min_len=min_len, prefix_id=prefix_id)
File “/home/dragon/soft/anaconda3/envs/metaphlan/lib/python3.7/site-packages/metaphlan/utils/read_fastx.py”, line 109, in read_and_write_raw_int
print_record(description + “__{}{}{}”.format(prefix_id, ‘.’ if prefix_id else ‘’, idx), sequence, qual, fmt))
BrokenPipeError: [Errno 32] Broken pipe

Hi @rooobben
It seems the error you are experimenting is due to lack of memory in your machine.