Hi all,
I am running metaphlan in conda environment in our HPC cluster. I am receiving the following error-
ValueError: End of file without quality information.
Background:
I have quality trimmed my metagenomics fastq files with bbduk and catenated the reverse and forward reads from Illumina HiSeq2500 V2 250x250bp runs.
When I am using the catenated fastq files as input, I receive the above error message.
My command:
bash
. ~/.bashrc
conda activate metaphlan
metaphlan --input_type fastq --bowtie2out 109Y --nproc 20 -o 109Y.tsv -s 109Y --biom 109Y --tmp_dir 109tmp cleaned_109Y.fq
Error details-
Appreciate any lead on this end.
Thanks