Hi, I’ve just tried your suggestion but it seems that now there’s some problem with Bowtie2:
Error message returned from metaphlan :
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
LANGUAGE = (unset),
LC_ALL = (unset),
LANG = “en_US.utf8”
are supported and installed on your system.
perl: warning: Falling back to the standard locale (“C”).
/home/juan.arboleda2/7_12_Metagenomics_training/miniconda3/envs/metabiome-humann/bin/bowtie2-align-s: error while loading shared libraries: libtbb.so.2: cannot open shared object file: No such file or directory
(ERR): Description of arguments failed!
Exiting now …
OSError: “Command ‘[‘bowtie2’, ‘-h’]’ returned non-zero exit status 127.”
Fatal error running BowTie2. Is BowTie2 in the system path?
Moreover, if I try to run bowtie2 --help
inside the conda environment I get a similar error message:
/home/juan.arboleda2/7_12_Metagenomics_training/miniconda3/envs/metabiome-humann/bin/bowtie2-align-s: error while loading shared libraries: libtbb.so.2: cannot open shared object file: No such file or directory
(ERR): Description of arguments failed!
Exiting now …
I found here that installing the Tbb library could solve the problem, however since I am not sudoer I installed it with conda install tbb
. After that it seems that Bowtie2 is working properly. I ran HUMAnN again and now it seems to be working fine, however the job has not ended yet, when it ends I will tell you if it was successful.
Thanks a lot for your help!