I’ve been using the LEfSe tool to get some candidate enzymatic reactions that are different in my treatment groups. However, it looks like at least one of the hits (with a pretty hefty estimated effect size) isn’t actually significant at all (see EC_3_2_1_23) in the attached figure.
The raw values for this are the following (5 ABT_3wo followed by 3 UT ):
EC_126.96.36.199 23601.13 22159.68 24630.1 25438.25 24401.71 22057.87 22046.91 28232.54
Since the first filter is a rank-sum test for significance, I’m struggling to understand how it came up as a false positive.
Thanks so much for your help.