Hi @garretta @LiaOb21 @rogasan @Peter_Janampa ,
Apologies that you are running into issues. This week was the yearly maintenance on our servers and it has affected our Galaxy site, we are working on resolving this issue ASAP. Our software folks will let you know when we have fixed this bug.
Alternatively, you can use MaAsLin 2, a newer tool of ours, to do a similar analysis to LEfSe if you need to run something quickly.
Best,
Kelsey