Humann3 error unable to unzip input fastq file

Hi I am unable to run humann function on my fastq file. I am not able to figure out the error I get.
I am able to run most of my fastq file but for few of them I am getting 2 types of error message. For few of them I get below meesage when I run below code:
Error 1:
(biobakery) [kots2z@bmi-460g10-05 fastq3]$ humann --input MP00064_Latha_merged.fastq.gz --output MP00064_Latha_merged_output
Creating output directory: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output
Output files will be written to: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output
Decompressing gzipped file …

Critical Error: Unable to unzip input file: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged.fastq.gz
CRITICAL ERROR: Unable to use gzipped input file. Please check the format of the input file.

I tried doing less input_file.fastq.gz and able to view it and nothing seems out of order.

Below is the log file that was created:
0/12/2022 10:21:29 AM - humann.humann - INFO: Running humann v3.0.0.alpha.4
10/12/2022 10:21:29 AM - humann.humann - INFO: Output files will be written to: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output
10/12/2022 10:21:29 AM - humann.humann - INFO: Writing temp files to directory: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output/MP00064_Latha_merged_humann_temp
10/12/2022 10:21:29 AM - humann.utilities - INFO: File ( /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged.fastq.gz ) is of format: fastq.gz
10/12/2022 10:21:29 AM - humann.utilities - INFO: Decompressing gzipped file …

For other few samples, I get below message:
Error 2:
(biobakery) [kots2z@bmi-460g10-05 fastq3]$ humann --input MP00064_Latha_merged.fastq.gz --output MP00064_Latha_merged_output
Creating output directory: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output
Output files will be written to: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged_output
Decompressing gzipped file …

Critical Error: Unable to unzip input file: /scratch/kots2z/staph_files_neg/fastq3/MP00064_Latha_merged.fastq.gz
CRITICAL ERROR: Unable to use gzipped input file. Please check the format of the input file.
(biobakery) [kots2z@bmi-460g10-05 fastq3]$ humann --input 75_NL_1_merged.fastq.gz --output 75_NL_1_merged_output
Creating output directory: /scratch/kots2z/staph_files_neg/fastq3/75_NL_1_merged_output
Output files will be written to: /scratch/kots2z/staph_files_neg/fastq3/75_NL_1_merged_output
Decompressing gzipped file …

Traceback (most recent call last):
File “/usr/local/anaconda3-2020/envs/biobakery/bin/humann”, line 33, in
sys.exit(load_entry_point(‘humann==3.0.0a4’, ‘console_scripts’, ‘humann’)())
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/site-packages/humann/humann.py”, line 907, in main
check_requirements(args)
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/site-packages/humann/humann.py”, line 619, in check_requirements
new_file=utilities.gunzip_file(args.input)
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/site-packages/humann/utilities.py”, line 250, in gunzip_file
shutil.copyfileobj(file_handle_gzip, file_handle)
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/shutil.py”, line 79, in copyfileobj
buf = fsrc.read(length)
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/gzip.py”, line 300, in read1
return self._buffer.read1(size)
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/_compression.py”, line 68, in readinto
data = self.read(len(byte_view))
File “/usr/local/anaconda3-2020/envs/biobakery/lib/python3.7/gzip.py”, line 482, in read
uncompress = self._decompressor.decompress(buf, size)
zlib.error: Error -3 while decompressing data: invalid distance too far back

Hello, Thank you for the detailed post. It looks like there might possibly be an issue with the compression of your input file. Try decompressing the file in full to get more information about the error. If you can totally decompress the file without error, try running HUMAnN again on the fastq file.

Thank you,
Lauren