HUMAnN 3.0: Error: Unexpected end of input files

Microbial community profiling HUMAnN
#1

Hi all,
I am running a single fastq file for HUMAnN in cluster with the following code:
conda activate humann
humann -i file.fq -o file_humann --threads 40

I am running this command on a cluster with 24CPUs and 50Gb memory with 40 threads.

I am getting the ERROR: Unexpected end of input files.
Could anyone please guide me in this? The error screenshot is pasted below-

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image1448×698 186 KB

#2

I tried running demo fastq file provided with HUMAnN, yet I am getting the similar error, might be something to do with my HUMAnN installation. Any leads? Error is thrown from Diamond

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image1624×954 117 KB