Hi, I am trying to run my fastq files with humann3. But I’m getting a critical error message when humann3 is attempting to run diamond. I have diamond 0.9.36 installed. I’m not sure why this is happening, could you help me? Thank you!
The error message:
Running diamond …
Aligning to reference database: uniref90_201901b_ec_filtered.dmnd
CRITICAL ERROR: Can not find file /oasis/tscc/scratch/yiz023/A033_10_fastq/A033_10_T0_merged_humann_temp/tmp2g66y6/diamond_m8_5Lv3R4
The command I’m using:
humann --input A033_10_T0_merged.fastq --output A033_10_fastq
The databases I downloaded:
humann_databases --download chocophlan full humann_dbs
Download URL: http://huttenhower.sph.harvard.edu/humann_data/chocophlan/full_chocophlan.v296_201901b.tar.gz
Downloading file of size: 16.01 GBhumann_databases --download uniref uniref90_ec_filtered_diamond humann_dbs
Download URL: http://huttenhower.sph.harvard.edu/humann_data/uniprot/uniref_ec_filtered/uniref90_ec_filtered_201901b_subset.tar.gz
Downloading file of size: 1.61 GB
UPDATE:
I tried again by running the demo file. This time it gave me another error saying cannot find executable diamond, but I do have diamond installed.
Decompressing gzipped file …
Running metaphlan …
Found g__Bacteroides.s__Bacteroides_dorei : 57.96% of mapped reads
Found g__Bacteroides.s__Bacteroides_vulgatus : 42.04% of mapped readsTotal species selected from prescreen: 2
Selected species explain 100.00% of predicted community composition
Creating custom ChocoPhlAn database …
Running bowtie2-build …
Running bowtie2 …
Total bugs from nucleotide alignment: 2
g__Bacteroides.s__Bacteroides_vulgatus: 1195 hits
g__Bacteroides.s__Bacteroides_dorei: 1260 hitsTotal gene families from nucleotide alignment: 545
Unaligned reads after nucleotide alignment: 88.3095238095 %
Running diamond …
Aligning to reference database: uniref90_demo_prots_v201901b.dmnd
CRITICAL ERROR: Can not find executable diamond