Hello,
I’m running:
% humann --version
humann v3.7
and I get an error that there’s a missing temp file (though there are other temp files in the indicated directory) when running on one of the examples:
% humann --input examples/demo.fasta.gz --output examples/output
Output files will be written to: /home/higleyadmin/humann_source/humann-master/examples/output
Decompressing gzipped file …
Running metaphlan …
Found t__SGB1815 : 57.46% of mapped reads ( s__Bacteroides_dorei,g__Bacteroides.s__Bacteroides_sp_3_1_33FAA,g__Bacteroides.s__Bacteroides_sp_AF25_18,g__Bacteroides.s__Bacteroides_sp_AF17_1,g__Bacteroides.s__Bacteroides_sp_AF39_10AT,g__Bacteroides.s__Bacteroides_sp_9_1_42FAA,g__Bacteroides.s__Bacteroides_sp_AM28_6,g__Bacteroides.s__Bacteroides_sp_AM18_9,g__Bacteroides.s__Bacteroides_sp_AM23_18,g__Bacteroides.s__Bacteroides_sp_AF16_29 )
Found t__SGB1814 : 42.54% of mapped reads ( s__Bacteroides_vulgatus,g__Phocaeicola.s__Phocaeicola_dorei,g__Phocaeicola.s__Phocaeicola_vulgatus,g__Bacteroides.s__Bacteroides_sp_AM27_13,g__Bacteroides.s__Bacteroides_sp_NMBE5,g__Bacteroides.s__Bacteroides_sp_3_1_40A,g__Bacteroides.s__Bacteroides_sp_AM26_11,g__Bacteroides.s__Bacteroides_sp_AF32_15BH,g__Bacteroides.s__Bacteroides_sp_4_3_47FAA )
Total species selected from prescreen: 21
Selected species explain 100.00% of predicted community composition
Creating custom ChocoPhlAn database …
Running bowtie2-build …
Running bowtie2 …
Total bugs from nucleotide alignment: 2
g__Bacteroides.s__Bacteroides_dorei: 1259 hits
g__Bacteroides.s__Bacteroides_vulgatus: 1289 hits
Total gene families from nucleotide alignment: 553
Unaligned reads after nucleotide alignment: 87.8666666667 %
Running diamond …
Aligning to reference database: uniref90_demo_prots_v201901b.dmnd
CRITICAL ERROR: Can not find file /home/higleyadmin/humann_source/humann-master/examples/output/demo_humann_temp/tmpvi0rtoj5/diamond_m8_jkg95ge_
Daniel