Hi @YUNPENG
Which database version did you use to run MetaPhlAn? If you used vJun23 you should be able to run HUMAnN 3.9, while if you used vOct22 you can use HUMAnN 4.alpha. The latest database version vJan25 still doesn’t have compatibility with HUMAnN.
Here are more details Humann 4 cant work with metaphlan 4 - #2 by franzosa
1 Like