Can Humann be run using proteins

Microbial community profiling HUMAnN
1

Hello everyone,

I hope everyone is doing well

I am doing my masters in metabolic potential for a large group of archaea species, yet I am very new to bioinformatics in general.

Therefore I was wondering if Humann can be run using protein sequences that I have obtained from annotating my species genomes using a combo of RAST+DRAM? Or can it only run on metagenomic/meta-transcript data?

Thank you very much

p.s if Humann is unsuited for this can someone please suggest alternatives as this would be so much appreciated

2

HUMAnN needs to start from meta’omic sequencing reads. It sounds like what you want is a genome annotation tool? We often use Prokka for that purpose.