Can Humann be run using proteins

Hello everyone,

I hope everyone is doing well

I am doing my masters in metabolic potential for a large group of archaea species, yet I am very new to bioinformatics in general.

Therefore I was wondering if Humann can be run using protein sequences that I have obtained from annotating my species genomes using a combo of RAST+DRAM? Or can it only run on metagenomic/meta-transcript data?

Thank you very much

p.s if Humann is unsuited for this can someone please suggest alternatives as this would be so much appreciated

HUMAnN needs to start from meta’omic sequencing reads. It sounds like what you want is a genome annotation tool? We often use Prokka for that purpose.