Hello,
I would like to calculate pathway abundances using a TSV file of RXN abundances (or UniRef90 abundances), following the HUMAnN v3.8 process. Actually, my RXN abundance file is derived from the standard HUMAnN workflow, but I have manually removed the abundance contributions from a specific species. I want to use this method to confirm the impact of that species on pathway abundances.
I understand that stratified pathway abundance can, to some extent, answer my question, but I believe the results from these two approaches should be different. Unfortunately, I haven’t found a similar functionality in HUMAnN. Could you please help me with this? Is there a way to achieve this within HUMAnN, or would I need to modify the code?
Thank you very much for your assistance!