The bioBakery help forum

Without any normalization and transformation, is the default LM equivalent to a parametric t-test?

Dear MaAsLin2 developer,

This might be a very silly question, I’ve been trying to understand how does MaAsLin2 work statistically, but as indicated in the title, if I don’t perform any normalization and transformation, and I only have 1 level of categorical variables to compare, the default linear model would just compare each variable with the reference using a linear model, which essentially makes it a parametric t-test, does this make sense? please correct me if I am wrong. In such a case, would MsAsLin2 tests for the assumptions of parametric tests before reporting the statistical conclusions?

Thank you!

Rui