The bioBakery help forum

ShortBRED installation/dependency errors

When I tried to run, I got following dependency errors: --goi input_prots.faa --ref ref_prots.faa --markers mytestmarkers.faa --tmp example_identify --usearch /usr/local/bin/usearch
Checking dependencies…
Traceback (most recent call last):
File “/opt/anaconda3/envs/py2/bin/”, line 156, in
File “/opt/anaconda3/envs/py2/bin/src/”, line 43, in CheckDependency
pCmd = subprocess.Popen([strCmd],stdout=subprocess.PIPE,stderr=subprocess.PIPE)
File “/opt/anaconda3/envs/py2/lib/python2.7/”, line 394, in init
errread, errwrite)
File “/opt/anaconda3/envs/py2/lib/python2.7/”, line 1047, in _execute_child
raise child_exception
OSError: [Errno 86] Bad CPU type in executable

Since it might be an issue due to 32bit support by Catalina, I switched to a lower version OSX. However, when I tried to install from conda, I got:

UnsatisfiableError: The following specifications were found
to be incompatible with the existing python installation in your environment:


  • shortbred -> python[version=’>=3.5,<3.6.0a0|>=3.6,<3.7.0a0|>=3.8,<3.9.0a0|>=3.7,<3.8.0a0’]

Your python: python=2.7

It looks like this ShortBRED conda install requires Python 3. If you’re already using conda, an easy way to solve this is to create a Python 3 environment and install ShortBRED into it.

$ conda create --name python3 python=3.7
$ conda activate python3
(python3) $ conda install -c biobakery shortbred