Question1: How can I adjust the labeling size of the heatmap legend (the one showing the range of values plotted on the top left corner of the png file). The font is too big causing the numbers to overlap with each other, and with the color legend.
My command is:
hclust2.py -i merged_abundance_table.txt -o merged_abundance2.png --f_dist_f braycurtis --s_dist_f braycurtis --cell_aspect_ratio 0.5 -l --ftop 50 --fperc 99 --flabel_size 4 --slabel_size 4 --max_flabel_len 100 --max_slabel_len 100 --minv 0.1 --dpi 300 --max_flabel_len 100 -c ‘Blues’ --colormap ‘Blues’
Question2: Are the abundance values scaled by row in hclust2?
Thanks
You can adjust the font size using the --colorbar_font_size parameter.
No, scaling is introduced with parameters -l or -s for log-scale and sqrt-scale, respectively.
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Another question, I am comparing two heatmaps with the same metadata. However, everytime I regenerated the heatmap, the color palette assigned to the metadata categories change. How do I manually specify which color goes with which metadata categories? I have two rows of metadata.
Best
Hi @Dahn-young_Dong , unfortunately, there is no option in hclust2 to specify the color for each metadata category, it will be chosen randomly