No UniRef90 IDs from Humann3 have information in UniProfKB site?

Microbial community profiling HUMAnN
1

Hi all,

I’m working from Hiseq data from mouse stool. I’ve gone through the process (actually, this forum has been a huge help since I’m a pretty inexperienced bioinformatician) and I finally have a list of genefamilies that I want to pursue. I’m trying to group them under different ontologies or somehow derive some general description from them and then pursue specific ones that relate to our hypotheses.

However, I couldn’t determine any sort of information about any of them from the UniProtKB database since it said that they were all ( all 63383 of them…) unmapped.

Does anyone have any experience with this? Below are some example UniRef IDs pasted below.

A9KLS5
A9KLZ7
A9KMA4
A9KN58
A9KN59
A9KN61
A9MN36
A9MQL6
A9MQQ3
B0G1T5
B0G2I0
B0G363
B0G460
B0G4I5
B0G4L8
B0G528
B0G539
C0H463
C0H465
C0K028
C0KTB1
C0KTB8
C0KTB9
C0KTC8
C0KTD3
C0KTD8

Thanks so much for your time.

2

When you say there were unmapped, are you referring to the UniProt ID mapping tool? Or were they possibly all “unclassified” in HUMAnN (which has a different meaning)?

You can look up individual UniProt IDs like this:

https://www.uniprot.org/uniprot/A9KLS5

We also package a bunch of utility mapping files with HUMAnN that can regroup UniRefs to broader protein families automatically (KOs, Pfams, etc.).

3

Whew, I thought I was going insane.

Thanks for the help, and I hate to ask such a basic question, but is there a way of doing this by pasting a list of IDs somewhere?

I have re-mapped the UniProd IDs to Enzyme comission IDs to test one of our hypothesis but the resulting spreadsheet only had like 9 EC IDs out of something like 500k UniProt IDs. Maybe that’s a separate issue?

Thanks again,