No differentially abundant features found in tax-otu-table-result.txt. although a number of significantly discriminative features is veryfied

Hi Mania,
I think this response will help with your question as well: Problme with LEfSe using command line interface - #5 by mishort
The commands you’re using are not the ones from the most updated version of LEfSe; if you update your version it should help.
Best,
Meg