Hello, I am trying to run the MetaPhlAn version 4.1.1 (11 Mar 2024) tutorial. For all the tutorial files, the tool is running perfectly, but for one file (SRS014459-Stool.fasta.gz), the tool is not able to detect any microbial taxa.
I downloaded the online profile.txt file (https://github.com/biobakery/MetaPhlAn/releases/download/4.0.2/SRS014459-Stool_profile.txt) given for the sample, and it does contain profiling data.
I am not sure where I am going wrong.
I tried running both the .fasta.gz and .fasta files and the results were the same.
I am attaching an image highlighting the command I ran and the output I received for profile and bowtie2 files.
Note: The tool ran successfully for all other example files, and the results were comparable to those of the online profile files.
Database used: #mpa_vJun23_CHOCOPhlAnSGB_202403
