Metacyc pathway and Species

Hi, Is it suitable to correlate Species and Metacyc Pathway (or GO)?
and Looking at the output, I see “1”, why is this happening?

To my understanding MetaCyc has some labelling of “this pathway is found in organisms X, Y, and Z” so it might make more sense to use that than to try and find where species abundance and pathway abundance correlate de novo.

The big one is showing up because all of your features clustered into a single large block. You can see that almost all of the cells in your plot have a dot in them, indicating that they were marginally significant. You should probably re-examine your FDR threshold and examine some of the pairwise feature plots of the strongest relationships.