Hello. We tried to perform the melonnpan-based metabolite prediction on our 16s sequencing raw data and we have come across a few doubts.
- Does Melonnpan accept the output from Picrust or from Humann2?
- If Melonnpan accepts Picrust output only, then how do we convert the KO gene IDs (produced by Picrust) to their respective Uniref90 IDs (input format of Melonnpan)?
- We tried giving our Picrust output to Humann2 to generate Uniref IDs, but Humann2 is only able to produce pathway abundances via a keggc- adducted route.
How can we bridge this gap in the Picrust (or Humann2) output files and the melonnpan input specifications?