Hello!
I am interested in integrating MACARRoN into my untargeted metabolomics workflow, especially for statistical analysis with repeated measures data.
Right now, we are using baseR packages in R studio to perform repeated measures one-way analysis of variance. Unfortunately, we have been unsuccessful in finding a way to create a looped code that works to generate a compiled list of posthoc/multiple comparisons testing for this analysis (which means that we can either 1) write a separate command line for each metabolite, or 2) perform posthoc testing in a different statistical analysis software)… neither option is ideal in terms of time management, as there are several hundred metabolites in the dataset.
We are curious if MACARRoN has the capacities to perform this type of analysis (repeated measures one-way ANOVA with posthoc comparisons- showing p-values, q-values, etc) with output that can readily be generated (simultaneously for hundreds of metabolites)?
A little more about the structure of our dataset:
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We have three experimental groups, and we are looking to compare each group over time (five timepoints: baseline/week 0, Week 6, Week 12, Week 18, Week 24)
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None of our subjects have missing data/timepoints
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We have 8 posthoc contrasts/comparisons that we want to include (each week versus week 0, selected weeks versus each other), to limit unnecessary contrasts in our statistical model.
Thank you very much for your time and input here!