Hi @himel.mallick - thank you for the prompt response. I see now. So you’re treating taxa as the dependent variable against a range of predictors. If I apply a ZINB with count data, is this different than running a ZINB using standard R packages or would it yield similar results under the same conditions? For example, would there be a difference between ZNIB used with MaAsLin 2 and the R packages ZNIB and NBZIMM?