The bioBakery help forum

MaAsLin2: possible with 2x4 samples?

Hello everyone,
I have a general statistics question and would be grateful if anyone had an answer! We have a shotgun metagenomics dataset of 2x4 samples and conducted MaAsLin2 analysis. However, we are really insecure whether this is valid for so few replicates. Can we use the results?
Thank you very much in advance and best regards,
Kat