The bioBakery help forum

Samples without control

Hi there,
Thanks for sharing your tool with us!

I am trying to use MMUPHin on my data for differential abundance for metagenomics
First i used the tutorial on th CRC data and it al worked fine

As i moved to my data - 5 sample of 3 responders and 2 nonresponders from a single study, i figured 2 things:

  1. i cant use it for a single study (i could solve it by changing my data for 2 studies)
  2. i cant use it without control (and i dont have control samples)

might be that i am missing something :slight_smile:
i will be happy to hear back from you!
Thanks

Hi -

Thanks for your interests! May I ask what your analysis goal is? It sounds like there are no batches/studies here, which is what MMUPHin is designed for. If you’re interested purely in differential abundance testing (comparing responders and non responders), I’d suggest checking out the lab’s MaAsLin2 tool.

Best,
Siyuan