Hello,
Currently I have two questions about running lefse
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I didn’t use the subclass option (i.e. my command looks like format_lefse.py ~.txt -c 1 -u 2 -o 1000000)
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Most importantly, I didn’t use the taxonomic hierarchy structure.
The purpose of analysis is just looking for specific taxa level marker(e.g. genus level) in 2 groups (e.g. CRC/healthy)
In this case, above two are still making lefse analysis valid??
Thank you.